Spray detection of phospholipids on thin-layer chromatograms.

Modified spray for the detection of phospholipids on thin-layer chromatograms.

A simplified method for the preparation of the Dittmer-Lester spray for the detection of phospholipids on thin-layer chromatograms is described.

A Simple, Specific Spray for the Detection of Phospholipids on Thin-layer Chromatograms.

Ferric chloride spray detector for cholesterol and cholesteryl esters on thin-layer chromatograms.

The use of a stable solution of ferric chloride for the detection of cholesterol and cholesteryl esters on thin-layer chromatographic layers is described. Its advantages over the commonly used Carr-Price reagent are listed, and the levels of sensitivity for the new reagent are shown. Detection of other lipid classes is also possible.

Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms.

A simple modification of the Dittmer-Lester reagent is described that allow the detection of phospholipid derivatives at very low concentrations on silica gel and reversed-phase thin-layer plates. This modification, which involves the addition of acetic acid to the mixture, permits the observation of sharp blue spots on a white background. The specificity and sensitivity of the spray are discus...

A novel method for the separation and identification of bile acids and phospholipids of bile on thin-layer chromatograms.

Cholesterol gallstone disease, so common in the western world, ‘has consider&le medical and economic importance. Under normal physiological conditions, cholesterol is held in solution in the bile. If the solubility of cholesterol in bile is changed, cholesterol crystals will precipitate and lead to the formation of gallstones. The solubility of cholesterol in bile depends on three major lipid c...